cgsc2.biology.yale.eduColi Genetic Stock Center

CGSC E. coli Genetic Resources at Yale CGSC, The Pay an invoice About the CGSC CGSC Home Scope of the Collection The Database Privacy Policy Acknowledgment of Support Searching the CGSC Database Help With Query Forms Strain Query Mutation Query Gene/Site Query Gene Product Query Reference Query Query Help Info on Genetic Nomenclature How to Request Strains or Information Contact Information Charges Reviving Strains Strains Not Found? Other CGSC Information FAQ on Procedures Current Working Map 1998 MMBR Map 1998 MMBR Gene List Map Diagrams (PDFs) Other Links E. coli Links Other Stock Collections Other Bio Links Yale Web Funded by a grant from the National Science Foundation CGSC DB-WebServer: Access To and Information About All Query Forms. Which Query form should I use? Strain Query Mutation Query Gene/Plasmid/Phage/Chrom.Sites Query Gene Product Query Reference Query Query Help Notice 1/1/2024 The CGSC at Yale has been discontinued, Strains from the CGSC collection are now available from the E. coli Genetic Resource Center . Questions and correspondence should be directed to john.wertz@ecgrc.net Notice 7/1/2023 Unfortunately due to limited resources, the CGSC has had to suspend the distribution of strains indefinetely. The collection is still being maintaned, and the web database will remain up as a resource to the bacterial genetics community. We hope to be able to resume strain distributions at some future date.Notice: The CGSC@yale.edu email address is currently down due to a hardware failure. Please send requests to the following email address: john.wertz@yale.edu until further notice. (posted 10/18/10)The Stock Center will be closed for the holiday season Dec 24 - Jan 3.The Stock Center will be closed for the holiday season Dec 24 - Jan 3.The Stock Center is moving to a new building on 8/20/2019. We will not be able to ship strains for the week before and after this date. We apologize for the disruption in service during this time. ***UPDATE 9/10/2019 We have resumed shipping strains this week. Please be patient while we work through the backlog of requests. There are still some remaining issues from the move. This webserver is scheduled to be moved Friday 9/13/2019, so there will also be a brief period when our website will be down while it is migrated to a new IP address. Also, the lab phone number is not working yet, so please use my office phone number if you need to contact me by phone.Due to changes in funding, effective March 15th 2014 there will be a $1 per strain increase in the distribution fee. here .Due to changes in funding, effective March 15th 2014 there will be a $1 per strain increase in the distribution fee.Links and lists of commonly requested and searched for strains Browsable list of YFP protein fusion Strains Browsable list of Keio Knockout Strains List of commonly requested strains auxotrophic for amino acids Strain kits for using the Wanner Lambda Red method of gene disruption TO REQUEST STRAINS or additional information, email us at CGSC@yale.edu or see our other contact information . When requesting strains please include the shipping address and the name of the Primary Investigator of the laboratory. A description of the charges for strains can be found here . INFORMATION ON HOW STRAINS ARE SHIPPED A description of our shipping methods and instructions on how to revive strains can be found here . Note: The CGSC Collection contains only non-pathogenic BSL-1 laboratory strains, primarily genetic derivatives of Escherichia coli K-12, the laboratory strain widely used in genetic and molecular studies. For details, see About CGSC in the navigation bar on the left. ssDNA Ladders ss100 DNA Ladder is a single-stranded DNA size marker for gel electrophoresis that gives bands at 100-nucleotide increments with sizes ranging from 100 to greater than 1000 nucleotides. NEW SIZES ss10 DNA Ladder is a single-stranded DNA size marker for gel electrophoresis that gives bands at 10-nucleotide increments with sizes ranging from 10 to 100 nucleotides. ss50 DNA Ladder is a single-stranded DNA size marker for gel electrophoresis that gives bands at 50-nucleotide increments with sizes ranging from 50 to greater than 1000 nucleotides. ss200 DNA Ladder is a single-stranded DNA size marker for gel electrophoresis that gives bands at 200-nucleotide increments with sizes ranging from 200 to greater than 3000 nucleotides.The CGSC Database of E. coli genetic information includes genotypes and reference information for the strains in the CGSC collection, the names, synonyms, properties, and map position for genes , gene product information, and information on specific mutations and references to primary literature . The public version of the database includes this information and can be queried directly via this CGSC DB WebServer. For help, use the help links located above and on each query form, or contact us directly...